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The Efficiency of Intrauterine Irrigation of the Cavitated Drug Solutes in Patients with Endometrial Receptivity Disorders

Received: 6 June 2018     Accepted: 12 July 2018     Published: 17 September 2018
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Abstract

Evaluate the efficiency of intrauterine irrigation of cavitated low-frequency sonication drug solution in restoring of endometrial receptivity in patients with uterine infertility. 92 women with uterine infertility due to endometrial hypoplasia were examined in the dynamics of therapy including irrigation of the uterine cavity with cavitated low-frequency ultrasound with drug solutions. The control group consisted of 28 healthy fertile women. An ultrasound examination of the pelvic organs with color dopplerogragy, histological and immunohistochemical study of the endometrium was performed on the "implantation window" LH + 7, in dynamics before the start of therapy and in the next cycle after the end of treatment. After cavitation irrigation of the uterine cavity, 62 (67.39%) women with infertility in the endometrial specimens had adequate vascularization of the stroma (before treatment, 36.95%, p=0.017, in control 78.57%), in 68 (73, 91%) women - a decrease in the density of the stromal matrix, in 64 (69.56%) - according to light microscopy data, mature pinopodia were determined (initially 28.26%, p=0.0015). On the background of therapy, the ratio of ERα / PR expression in the stroma of the implantation endometrium was normalized, which was characterized by the prevalence of the PR pool (1.01±0.34 before treatment, 0.36 ± 0.03 after therapy, p = 0.040, in control 0.34±0.06, p> 0.05). The parameters of stromal expression of natural uterine killers CD56 + bright against the background of treatment in patients with infertility come in accordance with the indices of healthy fertile women (from 35.21 ± 2.14 cells in l/s to 45.75±3.18 cells l/s; in the control 47.8±2.13 cells in l/s; p> 0.05). A significant decrease in stromal expression of CD3+ is noted: 78.17±6.89 cells in l/s versus 23.83±3.63 cells in l/s; p=0.004. The expression level of CD34 in the endometrial stroma after the treatment was significantly increased (20.88±0.77 cells in l/s versus 33.83±3.63 cells in l/s, p=0.035). The use of G-CSF cavitaion solutions for irrigation of the uterine cavity appears to be effective in restoring tissue and molecular endometrial receptivity in patients with uterine infertility due to endometrial hypoplasia.

Published in Journal of Gynecology and Obstetrics (Volume 6, Issue 4)
DOI 10.11648/j.jgo.20180604.15
Page(s) 98-107
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2018. Published by Science Publishing Group

Keywords

Uterine Infertility, Endometrial Hypoplasia, Cavitation Irrigation of Uterine Cavity, Granulocyte Colony-Stimulating Factor

References
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    Oxana Alexandrovna Melkozerova, Evgeniy Yuryevich Gluchov, Nadezda Vasilyevna Bashmakova, Guzel Nuchovna Chistyakova, Alena Valeryevna Lvova, et al. (2018). The Efficiency of Intrauterine Irrigation of the Cavitated Drug Solutes in Patients with Endometrial Receptivity Disorders. Journal of Gynecology and Obstetrics, 6(4), 98-107. https://doi.org/10.11648/j.jgo.20180604.15

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    ACS Style

    Oxana Alexandrovna Melkozerova; Evgeniy Yuryevich Gluchov; Nadezda Vasilyevna Bashmakova; Guzel Nuchovna Chistyakova; Alena Valeryevna Lvova, et al. The Efficiency of Intrauterine Irrigation of the Cavitated Drug Solutes in Patients with Endometrial Receptivity Disorders. J. Gynecol. Obstet. 2018, 6(4), 98-107. doi: 10.11648/j.jgo.20180604.15

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    Oxana Alexandrovna Melkozerova, Evgeniy Yuryevich Gluchov, Nadezda Vasilyevna Bashmakova, Guzel Nuchovna Chistyakova, Alena Valeryevna Lvova, et al. The Efficiency of Intrauterine Irrigation of the Cavitated Drug Solutes in Patients with Endometrial Receptivity Disorders. J Gynecol Obstet. 2018;6(4):98-107. doi: 10.11648/j.jgo.20180604.15

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  • @article{10.11648/j.jgo.20180604.15,
      author = {Oxana Alexandrovna Melkozerova and Evgeniy Yuryevich Gluchov and Nadezda Vasilyevna Bashmakova and Guzel Nuchovna Chistyakova and Alena Valeryevna Lvova and Anna Michaylovna Bogdanova and Aigul Adipovna Giniyatova and Olga Germanovna Barlit},
      title = {The Efficiency of Intrauterine Irrigation of the Cavitated Drug Solutes in Patients with Endometrial Receptivity Disorders},
      journal = {Journal of Gynecology and Obstetrics},
      volume = {6},
      number = {4},
      pages = {98-107},
      doi = {10.11648/j.jgo.20180604.15},
      url = {https://doi.org/10.11648/j.jgo.20180604.15},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.jgo.20180604.15},
      abstract = {Evaluate the efficiency of intrauterine irrigation of cavitated low-frequency sonication drug solution in restoring of endometrial receptivity in patients with uterine infertility. 92 women with uterine infertility due to endometrial hypoplasia were examined in the dynamics of therapy including irrigation of the uterine cavity with cavitated low-frequency ultrasound with drug solutions. The control group consisted of 28 healthy fertile women. An ultrasound examination of the pelvic organs with color dopplerogragy, histological and immunohistochemical study of the endometrium was performed on the "implantation window" LH + 7, in dynamics before the start of therapy and in the next cycle after the end of treatment. After cavitation irrigation of the uterine cavity, 62 (67.39%) women with infertility in the endometrial specimens had adequate vascularization of the stroma (before treatment, 36.95%, p=0.017, in control 78.57%), in 68 (73, 91%) women - a decrease in the density of the stromal matrix, in 64 (69.56%) - according to light microscopy data, mature pinopodia were determined (initially 28.26%, p=0.0015). On the background of therapy, the ratio of ERα / PR expression in the stroma of the implantation endometrium was normalized, which was characterized by the prevalence of the PR pool (1.01±0.34 before treatment, 0.36 ± 0.03 after therapy, p = 0.040, in control 0.34±0.06, p> 0.05). The parameters of stromal expression of natural uterine killers CD56 + bright against the background of treatment in patients with infertility come in accordance with the indices of healthy fertile women (from 35.21 ± 2.14 cells in l/s to 45.75±3.18 cells l/s; in the control 47.8±2.13 cells in l/s; p> 0.05). A significant decrease in stromal expression of CD3+ is noted: 78.17±6.89 cells in l/s versus 23.83±3.63 cells in l/s; p=0.004. The expression level of CD34 in the endometrial stroma after the treatment was significantly increased (20.88±0.77 cells in l/s versus 33.83±3.63 cells in l/s, p=0.035). The use of G-CSF cavitaion solutions for irrigation of the uterine cavity appears to be effective in restoring tissue and molecular endometrial receptivity in patients with uterine infertility due to endometrial hypoplasia.},
     year = {2018}
    }
    

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  • TY  - JOUR
    T1  - The Efficiency of Intrauterine Irrigation of the Cavitated Drug Solutes in Patients with Endometrial Receptivity Disorders
    AU  - Oxana Alexandrovna Melkozerova
    AU  - Evgeniy Yuryevich Gluchov
    AU  - Nadezda Vasilyevna Bashmakova
    AU  - Guzel Nuchovna Chistyakova
    AU  - Alena Valeryevna Lvova
    AU  - Anna Michaylovna Bogdanova
    AU  - Aigul Adipovna Giniyatova
    AU  - Olga Germanovna Barlit
    Y1  - 2018/09/17
    PY  - 2018
    N1  - https://doi.org/10.11648/j.jgo.20180604.15
    DO  - 10.11648/j.jgo.20180604.15
    T2  - Journal of Gynecology and Obstetrics
    JF  - Journal of Gynecology and Obstetrics
    JO  - Journal of Gynecology and Obstetrics
    SP  - 98
    EP  - 107
    PB  - Science Publishing Group
    SN  - 2376-7820
    UR  - https://doi.org/10.11648/j.jgo.20180604.15
    AB  - Evaluate the efficiency of intrauterine irrigation of cavitated low-frequency sonication drug solution in restoring of endometrial receptivity in patients with uterine infertility. 92 women with uterine infertility due to endometrial hypoplasia were examined in the dynamics of therapy including irrigation of the uterine cavity with cavitated low-frequency ultrasound with drug solutions. The control group consisted of 28 healthy fertile women. An ultrasound examination of the pelvic organs with color dopplerogragy, histological and immunohistochemical study of the endometrium was performed on the "implantation window" LH + 7, in dynamics before the start of therapy and in the next cycle after the end of treatment. After cavitation irrigation of the uterine cavity, 62 (67.39%) women with infertility in the endometrial specimens had adequate vascularization of the stroma (before treatment, 36.95%, p=0.017, in control 78.57%), in 68 (73, 91%) women - a decrease in the density of the stromal matrix, in 64 (69.56%) - according to light microscopy data, mature pinopodia were determined (initially 28.26%, p=0.0015). On the background of therapy, the ratio of ERα / PR expression in the stroma of the implantation endometrium was normalized, which was characterized by the prevalence of the PR pool (1.01±0.34 before treatment, 0.36 ± 0.03 after therapy, p = 0.040, in control 0.34±0.06, p> 0.05). The parameters of stromal expression of natural uterine killers CD56 + bright against the background of treatment in patients with infertility come in accordance with the indices of healthy fertile women (from 35.21 ± 2.14 cells in l/s to 45.75±3.18 cells l/s; in the control 47.8±2.13 cells in l/s; p> 0.05). A significant decrease in stromal expression of CD3+ is noted: 78.17±6.89 cells in l/s versus 23.83±3.63 cells in l/s; p=0.004. The expression level of CD34 in the endometrial stroma after the treatment was significantly increased (20.88±0.77 cells in l/s versus 33.83±3.63 cells in l/s, p=0.035). The use of G-CSF cavitaion solutions for irrigation of the uterine cavity appears to be effective in restoring tissue and molecular endometrial receptivity in patients with uterine infertility due to endometrial hypoplasia.
    VL  - 6
    IS  - 4
    ER  - 

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Author Information
  • Ural Research Institute of Maternity and Child Care Russian Ministry of Health, Ekaterinburg, Russia

  • Ural State Medical University Russian Ministry of Health, Ekaterinburg, Russia

  • Ural Research Institute of Maternity and Child Care Russian Ministry of Health, Ekaterinburg, Russia

  • Ural Research Institute of Maternity and Child Care Russian Ministry of Health, Ekaterinburg, Russia

  • Ural Research Institute of Maternity and Child Care Russian Ministry of Health, Ekaterinburg, Russia

  • Ural State Medical University Russian Ministry of Health, Ekaterinburg, Russia

  • Ural Research Institute of Maternity and Child Care Russian Ministry of Health, Ekaterinburg, Russia

  • Ural State Medical University Russian Ministry of Health, Ekaterinburg, Russia

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