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The Effect of Paclitaxel on the Viability of U14 Cells

Received: 18 April 2020     Accepted: 29 April 2020     Published: 11 May 2020
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Abstract

The research is aimed to study the effect of paclitaxel on the viability of U14 cell line of cervical cancer, and to provide new ideas for further exploring the mechanism of paclitaxel chemotherapy to cervical cancer. Then, different concentrations of paclitaxel were used to treat U14 cells of cervical cancer in logarithmic phase growth. After culturing these cells for 24h, 48h or 72h, MTT method and automatic enzyme-linked immunosorbent assay system were used to evaluate the survival rate of cultured cells with different concentrations of paclitaxel. These results showed that paclitaxel has a significant inhibitory effect on the proliferation of U14 cells in a concentration- and time-dependent manner, compared with the control group. Paclitaxel concentration of the IC50 of U14 cells was 168.8μg/ml at 24h, 22. 15 μg/ml at 48h and 8. 04μg/ml at 72 h. As the time increased, the IC50 value of U14 cells gradually decreased. The results of linear regression analysis are as follows: 24 h, y=-0.005x + 1.344, R2=0.779; 48 h, y=-0.013x +0.788, R2=0.923; 72 h, y=-0.056x + 0.950, R2=0.908. The dose-effect curve of paclitaxel on U14 cells for 48 h and the trend of linear regression fitting are better, and the growth inhibition of cells shows a clear relationship between time and dose. In conclusion, paclitaxel has an obvious inhibitory effect on the activity of cervical cancer U14 cells in mice, which provides new ideas for further exploring the mechanism of cervical cancer paclitaxel chemotherapy.

Published in Journal of Gynecology and Obstetrics (Volume 8, Issue 3)
DOI 10.11648/j.jgo.20200803.11
Page(s) 51-54
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2020. Published by Science Publishing Group

Keywords

Paclitaxel, Cervical Cancer, Cell Viability, U14 Cell

References
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[2] Song B, Ding C, Chen W, Sun H, Zhang M, Chen W. Incidence and mortality of cervical cancer in China, 2013. Chinese journal of cancer research 2017, 29 (6): 471-476.
[3] Yang K, Park W, Huh SJ, Bae DS, Kim BG, Lee JW. Clinical outcomes in patients treated with radiotherapy after surgery for cervical cancer. Radiation oncology journal 2017, 35 (1): 39-47.
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[6] Naaz F, Haider MR, Shafi S, Yar MS. Anti-tubulin agents of natural origin: Targeting paclitaxel, vinca, and colchicine binding domains. European journal of medicinal chemistry 2019, 171: 310-331.
[7] Chavez JD, Keller A, Zhou B, Tian R, Bruce JE. Cellular Interactome Dynamics during Paclitaxel Treatment. Cell reports 2019, 29 (8): 2371-2383 e2375.
[8] Wang H, Zhu Y, Hao C, Fan J, Liu Y, Wang Y. Establishment of a drug-resistant human cervical cancer cell line and research on its drug-resistance. Journal of cancer research and therapeutics 2019, 15 (6): 1221-1225.
[9] Lee BE, Choi BY, Hong DK, Kim JH, Lee SH, Kho AR, Kim H, Choi HC, Suh SW. The cancer chemotherapeutic agent paclitaxel (Paclitaxel) reduces hippocampal neurogenesis via down-regulation of vesicular zinc. Scientific reports 2017, 7 (1): 11667.
[10] Aborehab NM, Osama N. Effect of Gallic acid in potentiating chemotherapeutic effect of Paclitaxel in HeLa cervical cancer cells. Cancer cell international 2019, 19: 154.
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  • APA Style

    Gao Xuesong, Lin Shaoqiang, Wang Xiaoyu. (2020). The Effect of Paclitaxel on the Viability of U14 Cells. Journal of Gynecology and Obstetrics, 8(3), 51-54. https://doi.org/10.11648/j.jgo.20200803.11

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    ACS Style

    Gao Xuesong; Lin Shaoqiang; Wang Xiaoyu. The Effect of Paclitaxel on the Viability of U14 Cells. J. Gynecol. Obstet. 2020, 8(3), 51-54. doi: 10.11648/j.jgo.20200803.11

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    AMA Style

    Gao Xuesong, Lin Shaoqiang, Wang Xiaoyu. The Effect of Paclitaxel on the Viability of U14 Cells. J Gynecol Obstet. 2020;8(3):51-54. doi: 10.11648/j.jgo.20200803.11

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  • @article{10.11648/j.jgo.20200803.11,
      author = {Gao Xuesong and Lin Shaoqiang and Wang Xiaoyu},
      title = {The Effect of Paclitaxel on the Viability of U14 Cells},
      journal = {Journal of Gynecology and Obstetrics},
      volume = {8},
      number = {3},
      pages = {51-54},
      doi = {10.11648/j.jgo.20200803.11},
      url = {https://doi.org/10.11648/j.jgo.20200803.11},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.jgo.20200803.11},
      abstract = {The research is aimed to study the effect of paclitaxel on the viability of U14 cell line of cervical cancer, and to provide new ideas for further exploring the mechanism of paclitaxel chemotherapy to cervical cancer. Then, different concentrations of paclitaxel were used to treat U14 cells of cervical cancer in logarithmic phase growth. After culturing these cells for 24h, 48h or 72h, MTT method and automatic enzyme-linked immunosorbent assay system were used to evaluate the survival rate of cultured cells with different concentrations of paclitaxel. These results showed that paclitaxel has a significant inhibitory effect on the proliferation of U14 cells in a concentration- and time-dependent manner, compared with the control group. Paclitaxel concentration of the IC50 of U14 cells was 168.8μg/ml at 24h, 22. 15 μg/ml at 48h and 8. 04μg/ml at 72 h. As the time increased, the IC50 value of U14 cells gradually decreased. The results of linear regression analysis are as follows: 24 h, y=-0.005x + 1.344, R2=0.779; 48 h, y=-0.013x +0.788, R2=0.923; 72 h, y=-0.056x + 0.950, R2=0.908. The dose-effect curve of paclitaxel on U14 cells for 48 h and the trend of linear regression fitting are better, and the growth inhibition of cells shows a clear relationship between time and dose. In conclusion, paclitaxel has an obvious inhibitory effect on the activity of cervical cancer U14 cells in mice, which provides new ideas for further exploring the mechanism of cervical cancer paclitaxel chemotherapy.},
     year = {2020}
    }
    

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  • TY  - JOUR
    T1  - The Effect of Paclitaxel on the Viability of U14 Cells
    AU  - Gao Xuesong
    AU  - Lin Shaoqiang
    AU  - Wang Xiaoyu
    Y1  - 2020/05/11
    PY  - 2020
    N1  - https://doi.org/10.11648/j.jgo.20200803.11
    DO  - 10.11648/j.jgo.20200803.11
    T2  - Journal of Gynecology and Obstetrics
    JF  - Journal of Gynecology and Obstetrics
    JO  - Journal of Gynecology and Obstetrics
    SP  - 51
    EP  - 54
    PB  - Science Publishing Group
    SN  - 2376-7820
    UR  - https://doi.org/10.11648/j.jgo.20200803.11
    AB  - The research is aimed to study the effect of paclitaxel on the viability of U14 cell line of cervical cancer, and to provide new ideas for further exploring the mechanism of paclitaxel chemotherapy to cervical cancer. Then, different concentrations of paclitaxel were used to treat U14 cells of cervical cancer in logarithmic phase growth. After culturing these cells for 24h, 48h or 72h, MTT method and automatic enzyme-linked immunosorbent assay system were used to evaluate the survival rate of cultured cells with different concentrations of paclitaxel. These results showed that paclitaxel has a significant inhibitory effect on the proliferation of U14 cells in a concentration- and time-dependent manner, compared with the control group. Paclitaxel concentration of the IC50 of U14 cells was 168.8μg/ml at 24h, 22. 15 μg/ml at 48h and 8. 04μg/ml at 72 h. As the time increased, the IC50 value of U14 cells gradually decreased. The results of linear regression analysis are as follows: 24 h, y=-0.005x + 1.344, R2=0.779; 48 h, y=-0.013x +0.788, R2=0.923; 72 h, y=-0.056x + 0.950, R2=0.908. The dose-effect curve of paclitaxel on U14 cells for 48 h and the trend of linear regression fitting are better, and the growth inhibition of cells shows a clear relationship between time and dose. In conclusion, paclitaxel has an obvious inhibitory effect on the activity of cervical cancer U14 cells in mice, which provides new ideas for further exploring the mechanism of cervical cancer paclitaxel chemotherapy.
    VL  - 8
    IS  - 3
    ER  - 

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Author Information
  • Department of Obstetrics and Gynecology, The First Affiliated Hospital of Jinan University, Guangzhou, China

  • The Hospital Management Department of Guangdong Pharmaceutical University, Guangzhou, China

  • Department of Obstetrics and Gynecology, The First Affiliated Hospital of Jinan University, Guangzhou, China

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