Effects of <i>Buyang Huanwu</i> Decoction on Endoplasmic Reticulum Stress-Autophagy Response and GADD34 Expression in Mice with CIRI

Zhou Shengqiang; Li Bo; Liu Fang

doi:doi:10.11648/j.sd.20210906.33

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Effects of Buyang Huanwu Decoction on Endoplasmic Reticulum Stress-Autophagy Response and GADD34 Expression in Mice with CIRI

Zhou Shengqiang, Li Bo, Liu Fang

Published in Science Discovery (Volume 9, Issue 6)

Received: 19 November 2021 Accepted: 6 December 2021 Published: 7 December 2021

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Abstract

Objective: To investigate the effects of Buyang Huanwu Decoction on endoplasmic reticulum stress-autophagic response and the expression of GADD34 in ischemic penumbra of mice with CIRI. Methods: The mice were randomly divided into sham operation group, model group and Buyang Huanwu Decoction low-, medium-, and high-dosage groups. The mice were treated by gavage for 7 days before modeling and 24 hours after modeling. CIRI model was established by the thread embolization method. The neurological function was evaluated on the 48 hours after modeling; TTC staining was used to observe the cerebral infarct volume; HE staining was used to observe the pathological changes of ischemic penumbra; TUNEL was performed to assess cell apoptosis; Western blot was performed to assess the expression of endoplasmic reticulum stress marker protein GRP78 and autophagy marker proteins LC3 and p62; Immunohistochemistry and real-time PCR were applied to examine the protein and mRNA expressions of GADD34. Results: Compared with the model group, neurological function score and volume of cerebral infarction were decreased, reperfusion injury of ischemic penumbra was improved with varying degrees, number of apoptotic cells was decreased, expression of GRP78 and p62 protein were down-regulated while ratio of LC3-II/ LC3 –I was up-regulated, and expression of GADD34 protein and mRNA were up-regulated in each Buyang Huanwu Decoction group, with statistical significance (P<0.01). Conclusion Buyang Huanwu Decoction can significantly improve the damaged neurological function, cerebral infarction volume and histopathological damage of ischemic penumbra, as well as reduce the apoptosis cells, which plays a neuroprotective role in mice with CIRI. Its mechanism may be related to up regulating the expression of GADD34, regulating the steady-state balance of endoplasmic reticulum stress autophagy response in ischemic penumbra.

Published in	Science Discovery (Volume 9, Issue 6)
DOI	10.11648/j.sd.20210906.33
Page(s)	417-423
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2021. Published by Science Publishing Group

Keywords

Buyang Huanwu Decoction, Cerebral Ischemia-reperfusion Injury, Endoplasmic Reticulum Stress-autophagic Response, Growth Arrest and DNA Damage-inducible Protein 34, Mice

References

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APA Style

Zhou Shengqiang, Li Bo, Liu Fang. (2021). Effects of Buyang Huanwu Decoction on Endoplasmic Reticulum Stress-Autophagy Response and GADD34 Expression in Mice with CIRI. Science Discovery, 9(6), 417-423. https://doi.org/10.11648/j.sd.20210906.33

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ACS Style

Zhou Shengqiang; Li Bo; Liu Fang. Effects of Buyang Huanwu Decoction on Endoplasmic Reticulum Stress-Autophagy Response and GADD34 Expression in Mice with CIRI. Sci. Discov. 2021, 9(6), 417-423. doi: 10.11648/j.sd.20210906.33

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AMA Style

Zhou Shengqiang, Li Bo, Liu Fang. Effects of Buyang Huanwu Decoction on Endoplasmic Reticulum Stress-Autophagy Response and GADD34 Expression in Mice with CIRI. Sci Discov. 2021;9(6):417-423. doi: 10.11648/j.sd.20210906.33

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@article{10.11648/j.sd.20210906.33,
  author = {Zhou Shengqiang and Li Bo and Liu Fang},
  title = {Effects of Buyang Huanwu Decoction on Endoplasmic Reticulum Stress-Autophagy Response and GADD34 Expression in Mice with CIRI},
  journal = {Science Discovery},
  volume = {9},
  number = {6},
  pages = {417-423},
  doi = {10.11648/j.sd.20210906.33},
  url = {https://doi.org/10.11648/j.sd.20210906.33},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.sd.20210906.33},
  abstract = {Objective: To investigate the effects of Buyang Huanwu Decoction on endoplasmic reticulum stress-autophagic response and the expression of GADD34 in ischemic penumbra of mice with CIRI. Methods: The mice were randomly divided into sham operation group, model group and Buyang Huanwu Decoction low-, medium-, and high-dosage groups. The mice were treated by gavage for 7 days before modeling and 24 hours after modeling. CIRI model was established by the thread embolization method. The neurological function was evaluated on the 48 hours after modeling; TTC staining was used to observe the cerebral infarct volume; HE staining was used to observe the pathological changes of ischemic penumbra; TUNEL was performed to assess cell apoptosis; Western blot was performed to assess the expression of endoplasmic reticulum stress marker protein GRP78 and autophagy marker proteins LC3 and p62; Immunohistochemistry and real-time PCR were applied to examine the protein and mRNA expressions of GADD34. Results: Compared with the model group, neurological function score and volume of cerebral infarction were decreased, reperfusion injury of ischemic penumbra was improved with varying degrees, number of apoptotic cells was decreased, expression of GRP78 and p62 protein were down-regulated while ratio of LC3-II/ LC3 –I was up-regulated, and expression of GADD34 protein and mRNA were up-regulated in each Buyang Huanwu Decoction group, with statistical significance (PConclusion Buyang Huanwu Decoction can significantly improve the damaged neurological function, cerebral infarction volume and histopathological damage of ischemic penumbra, as well as reduce the apoptosis cells, which plays a neuroprotective role in mice with CIRI. Its mechanism may be related to up regulating the expression of GADD34, regulating the steady-state balance of endoplasmic reticulum stress autophagy response in ischemic penumbra.},
 year = {2021}
}

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TY  - JOUR
T1  - Effects of Buyang Huanwu Decoction on Endoplasmic Reticulum Stress-Autophagy Response and GADD34 Expression in Mice with CIRI
AU  - Zhou Shengqiang
AU  - Li Bo
AU  - Liu Fang
Y1  - 2021/12/07
PY  - 2021
N1  - https://doi.org/10.11648/j.sd.20210906.33
DO  - 10.11648/j.sd.20210906.33
T2  - Science Discovery
JF  - Science Discovery
JO  - Science Discovery
SP  - 417
EP  - 423
PB  - Science Publishing Group
SN  - 2331-0650
UR  - https://doi.org/10.11648/j.sd.20210906.33
AB  - Objective: To investigate the effects of Buyang Huanwu Decoction on endoplasmic reticulum stress-autophagic response and the expression of GADD34 in ischemic penumbra of mice with CIRI. Methods: The mice were randomly divided into sham operation group, model group and Buyang Huanwu Decoction low-, medium-, and high-dosage groups. The mice were treated by gavage for 7 days before modeling and 24 hours after modeling. CIRI model was established by the thread embolization method. The neurological function was evaluated on the 48 hours after modeling; TTC staining was used to observe the cerebral infarct volume; HE staining was used to observe the pathological changes of ischemic penumbra; TUNEL was performed to assess cell apoptosis; Western blot was performed to assess the expression of endoplasmic reticulum stress marker protein GRP78 and autophagy marker proteins LC3 and p62; Immunohistochemistry and real-time PCR were applied to examine the protein and mRNA expressions of GADD34. Results: Compared with the model group, neurological function score and volume of cerebral infarction were decreased, reperfusion injury of ischemic penumbra was improved with varying degrees, number of apoptotic cells was decreased, expression of GRP78 and p62 protein were down-regulated while ratio of LC3-II/ LC3 –I was up-regulated, and expression of GADD34 protein and mRNA were up-regulated in each Buyang Huanwu Decoction group, with statistical significance (PConclusion Buyang Huanwu Decoction can significantly improve the damaged neurological function, cerebral infarction volume and histopathological damage of ischemic penumbra, as well as reduce the apoptosis cells, which plays a neuroprotective role in mice with CIRI. Its mechanism may be related to up regulating the expression of GADD34, regulating the steady-state balance of endoplasmic reticulum stress autophagy response in ischemic penumbra.
VL  - 9
IS  - 6
ER  -

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Author Information

Zhou Shengqiang

Affiliated Hospital, Hunan Academy of Chinese Medicine, Changsha, China
Li Bo

The First Affiliated Hospital, Hunan University of Chinese Medicine, Changsha, China
Liu Fang

Affiliated Hospital, Hunan Academy of Chinese Medicine, Changsha, China

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APA Style

Zhou Shengqiang, Li Bo, Liu Fang. (2021). Effects of Buyang Huanwu Decoction on Endoplasmic Reticulum Stress-Autophagy Response and GADD34 Expression in Mice with CIRI. Science Discovery, 9(6), 417-423. https://doi.org/10.11648/j.sd.20210906.33

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ACS Style

Zhou Shengqiang; Li Bo; Liu Fang. Effects of Buyang Huanwu Decoction on Endoplasmic Reticulum Stress-Autophagy Response and GADD34 Expression in Mice with CIRI. Sci. Discov. 2021, 9(6), 417-423. doi: 10.11648/j.sd.20210906.33

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AMA Style

Zhou Shengqiang, Li Bo, Liu Fang. Effects of Buyang Huanwu Decoction on Endoplasmic Reticulum Stress-Autophagy Response and GADD34 Expression in Mice with CIRI. Sci Discov. 2021;9(6):417-423. doi: 10.11648/j.sd.20210906.33

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@article{10.11648/j.sd.20210906.33,
  author = {Zhou Shengqiang and Li Bo and Liu Fang},
  title = {Effects of Buyang Huanwu Decoction on Endoplasmic Reticulum Stress-Autophagy Response and GADD34 Expression in Mice with CIRI},
  journal = {Science Discovery},
  volume = {9},
  number = {6},
  pages = {417-423},
  doi = {10.11648/j.sd.20210906.33},
  url = {https://doi.org/10.11648/j.sd.20210906.33},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.sd.20210906.33},
  abstract = {Objective: To investigate the effects of Buyang Huanwu Decoction on endoplasmic reticulum stress-autophagic response and the expression of GADD34 in ischemic penumbra of mice with CIRI. Methods: The mice were randomly divided into sham operation group, model group and Buyang Huanwu Decoction low-, medium-, and high-dosage groups. The mice were treated by gavage for 7 days before modeling and 24 hours after modeling. CIRI model was established by the thread embolization method. The neurological function was evaluated on the 48 hours after modeling; TTC staining was used to observe the cerebral infarct volume; HE staining was used to observe the pathological changes of ischemic penumbra; TUNEL was performed to assess cell apoptosis; Western blot was performed to assess the expression of endoplasmic reticulum stress marker protein GRP78 and autophagy marker proteins LC3 and p62; Immunohistochemistry and real-time PCR were applied to examine the protein and mRNA expressions of GADD34. Results: Compared with the model group, neurological function score and volume of cerebral infarction were decreased, reperfusion injury of ischemic penumbra was improved with varying degrees, number of apoptotic cells was decreased, expression of GRP78 and p62 protein were down-regulated while ratio of LC3-II/ LC3 –I was up-regulated, and expression of GADD34 protein and mRNA were up-regulated in each Buyang Huanwu Decoction group, with statistical significance (PConclusion Buyang Huanwu Decoction can significantly improve the damaged neurological function, cerebral infarction volume and histopathological damage of ischemic penumbra, as well as reduce the apoptosis cells, which plays a neuroprotective role in mice with CIRI. Its mechanism may be related to up regulating the expression of GADD34, regulating the steady-state balance of endoplasmic reticulum stress autophagy response in ischemic penumbra.},
 year = {2021}
}

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TY  - JOUR
T1  - Effects of Buyang Huanwu Decoction on Endoplasmic Reticulum Stress-Autophagy Response and GADD34 Expression in Mice with CIRI
AU  - Zhou Shengqiang
AU  - Li Bo
AU  - Liu Fang
Y1  - 2021/12/07
PY  - 2021
N1  - https://doi.org/10.11648/j.sd.20210906.33
DO  - 10.11648/j.sd.20210906.33
T2  - Science Discovery
JF  - Science Discovery
JO  - Science Discovery
SP  - 417
EP  - 423
PB  - Science Publishing Group
SN  - 2331-0650
UR  - https://doi.org/10.11648/j.sd.20210906.33
AB  - Objective: To investigate the effects of Buyang Huanwu Decoction on endoplasmic reticulum stress-autophagic response and the expression of GADD34 in ischemic penumbra of mice with CIRI. Methods: The mice were randomly divided into sham operation group, model group and Buyang Huanwu Decoction low-, medium-, and high-dosage groups. The mice were treated by gavage for 7 days before modeling and 24 hours after modeling. CIRI model was established by the thread embolization method. The neurological function was evaluated on the 48 hours after modeling; TTC staining was used to observe the cerebral infarct volume; HE staining was used to observe the pathological changes of ischemic penumbra; TUNEL was performed to assess cell apoptosis; Western blot was performed to assess the expression of endoplasmic reticulum stress marker protein GRP78 and autophagy marker proteins LC3 and p62; Immunohistochemistry and real-time PCR were applied to examine the protein and mRNA expressions of GADD34. Results: Compared with the model group, neurological function score and volume of cerebral infarction were decreased, reperfusion injury of ischemic penumbra was improved with varying degrees, number of apoptotic cells was decreased, expression of GRP78 and p62 protein were down-regulated while ratio of LC3-II/ LC3 –I was up-regulated, and expression of GADD34 protein and mRNA were up-regulated in each Buyang Huanwu Decoction group, with statistical significance (PConclusion Buyang Huanwu Decoction can significantly improve the damaged neurological function, cerebral infarction volume and histopathological damage of ischemic penumbra, as well as reduce the apoptosis cells, which plays a neuroprotective role in mice with CIRI. Its mechanism may be related to up regulating the expression of GADD34, regulating the steady-state balance of endoplasmic reticulum stress autophagy response in ischemic penumbra.
VL  - 9
IS  - 6
ER  -

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